Methods Epithelial cells were isolated from sample tissue by cold trypsinization and cultured in serum-free growth medium.

方法 标本组织块用冷消化法分离出尿路上皮，养于血清养基。

Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.

方法取大鼠胚胎分离成纤维,分别采用悬液法和胰酶消化植块养法对其进行养。