But, there were extremely low efficiency of integration, unstable hereditation and expression in transgenic animals by the technique.
但是,这种方法存在基因整合效率低、外源基因不能在转基因动物体内稳定遗传及表达问题。
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Beginning with the lab of Paul Berg in 1972 (aided by EcoRI from Herbert Boyer's lab, building on work with ligase by Arthur Kornberg's lab), molecular biologists put these pieces together to produce the first transgenic organisms.
从1972年Paul Berg实验室开始(在Herbert Boyer实验室
EcoRI
帮助下,Arthur Kornberg
实验室利用连接酶进行工作),分子生物学家
这些碎片放在一起,生
了第一批转基因生物。