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Result Both mutanolysin and lysozyme could successfully lyse the wall of bifidobacterium and extract the plasmid from Bifidobacterium.

结果球孢发酵液与溶提取到质粒。

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Method Bifidobacterium plasmid was extracted by mutanolysin from Streptomyces globisporus which could lyse the wall of bifidobacterium.The result was compared with that by lysozyme.

法利用球孢发酵产生的变溶素来消化双歧杆细胞壁,提取质粒,并与商业化溶酶法比较。

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Some are exchanged via small loops of DNA called plasmids.

一些基因会通过被称为质粒的DNA小环交换。

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Osmosis-微生物

Additionally to the genetic material contained within the nucleoid, many bacteria contain extra chromosomal DNA molecules called plasmids.

在拟核的遗传物质外,许多细菌有额外的DNA分子,称为质粒。

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Did You Know? – Verge Science

Then they dropped the plasmid into a living bacterium, or yeast.

然后他们将质粒放入活细菌或酵母

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Osmosis-微生物

However, plasmids often contain genes that confer a selective advantage to the bacterium such as resistance to antibiotics.

不过质粒通常赋予细菌选择性优势的基因,例如抗生素耐药性。

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Osmosis-微生物

These plasmids are small double-stranded DNA molecules that can exist independently of the chromosome and they can be circular or linear.

它们是双链的DNA小分子,可以独立于细菌染体(拟核)而存在,可以是环状或线状的。

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2017级专外有声

Transfer of the ligated plasmid into an E. coli strain (transformation).

7。转化成功的细菌的筛选。

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Life Noggin

So essentially, these plasmids are the vehicles that transfer genes for antibiotic resistance between different bacterial species.

因此,从本质上讲,这些质粒是在不同细菌物种之间转移抗生素抗性基因的载体。

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SciShow 合集

This plasmid mutation has been seen in other places before; in China, in animals and some human patients.

这种质粒突变以前在其他地方也见过;在国,在动物和一些人类

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Life Noggin

The plasmid enters and multiplies itself within a cell and can then be transferred to others.

质粒进入细胞并在细胞内繁殖, 然后可以转移到其他细胞

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2017级专外有声

The cDNAs had to be linked to prokaryotic transcription and translation signals and inserted into multicopy plasmids.

然而,占总蛋白30%的过量表达的蛋白质是沉淀或不溶的形式并且缺少翻译后修饰。

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Osmosis-微生物

Plasmids have relatively few genes and their genetic information is not essential to the bacterium and cells that lack them usually function normally.

质粒所的基因相对较少,并且它们的基因对细菌来说并不是必须的,缺少质粒的细胞往往也可以正常生长。

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SciShow 合集

But this is the first time the plasmid has been found in the United States, and it could get passed around quickly.

但这是第一次在美国发现这种质粒,它可能会很快传播开来。

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2017级专外有声

More specialized vectors have been engineered to use aspects of plasmids and bacteriophages, such as the plasmid-bacteriophage h hybrids known as cosmids

质粒和噬菌体已经在很多种类的细菌被使用来表达基因,并且一些噬菌体也许已经被用来将DNA整合到宿主基因组上。

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Life Noggin

Or, in plain english, plasmids are part of the reason that, one day, antibiotics may not be able to help your strep throat anymore.

,用简单的英语来说,质粒是有一天抗生素可能无法再帮助您治疗链球菌性咽喉炎的部分原因。

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2017级专外有声

Phage h can be used to clone fragments larger than plasmid vectors, and phage M13 allows cloned DNA to be isolated in single-stranded form.

更多载体被改造能同时拥有质粒和噬菌体的一些特性,像被称为粘粒的质粒-噬菌体λ杂交体。

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2022诺贝尔得主访谈录

I did all these experiments actually, with my own hands, I was 58 years old, I was still culturing plasmids and feeding cells, so it is very unlikely.

实际上,所有这些实验都是我亲手做的,当时我 58 岁,我还在培养质粒和喂养细胞,所以这是非常不可能的。

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Osmosis-微生物

The cytoplasm contains ribosomes which are the site of protein synthesis, the nucleoid which contains the genetic material, and plasmids which contain genes that confer a selective advantage to the bacterium.

细胞质有核糖体,它们是蛋白质合成的部位;拟核有遗传物质以及质粒;质粒有赋予细菌选择性优势的基因。

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SciShow 合集

And the thing that makes this mutation especially troubling is that it was found on a plasmid -- a little piece of DNA that can be easily shared between neighboring bacteria, meaning it can spread more easily.

让这种突变特别麻烦的是它是在质粒上发现的——一小段 DNA 可以很容易地在相邻细菌之间共享,这意味着它可以更容易地传播。

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