The biruet can develop the color with the cupric sulfate in the alkaline solution, we call that the biruet reaction.Malonyl urea has acidity, so we call it barbituric acid.

巴比妥酸本身无镇静作用，五位亚甲基上两个氢烃基取代后才呈现镇静生理活性，是一类对中枢神经系统起抑制作用化合物。