Objective To investigate the feasibility of applying immunohistochemistry (IH) and in situ hybridization (ISH) technique to the undecalcified bone sections.
Many modern molecular tests such as flow cytometry, polymerase chain reaction (PCR), In situ hybridization, immunohistochemistry, cytogenetics and gene rearrangements studies fall within the territory of pathology.
Methods c-Fos expression was evaluated by immunohistochemistry on articular cartilage sections from patients with OA and mice subjected to the destabilisation of the medial meniscus (DMM) model of OA.