The first step is denaturation, then there's annealing, and finally extension.

第一步是变性，然后是退火，后是延伸。

Taq polymerase is used to amplify the cDNA through denaturation, annealing and extension steps.

Taq合通过变性、退火、延伸等步骤用于扩增cDNA。

So not only can Taq polymerase withstand the heat during the denaturation step of PCR, but it also functions best at around 72 degrees Celsius.

因此，Taq合不仅能承受PCR变性步骤中的热量，而且在72摄氏度左右能发好的作用。

These alcohols can react with different parts of many proteins, disrupting the bonds that hold them together, and ultimately breaking them down in a process known as denaturation.

这些酒精可以和很多蛋白质的不同部分产生反应，破坏将它们结合在一起的化学键，并终将其分解，这一过程称为变性。