A method for fluorophotometric assay of dihydrofolate reductasc (DHFR) activity by the use of fluorescence properties of dihydrofolate (DHFA) and NADPH was developed.
In the first stage, the energized electrons from chlorophyll are used to create two vital energy--carrying molecules: ATP (adenosine triphosphate) and NADPH (nicotinamide adenine dinucleotide phosphate).