The amino group becomes attached to the coenzyme to form pyridoxamine phosphate, and is then transferred to the a-keto acid, which is usually pyruvic acid, oxaloacetic acid, or α-ketoglutaric acid.
反应中基首先连接在辅酶上形成磷酸吡哆,然后基转移到α-酮酸上,这种α-酮酸可以为丙酮酸、草酰乙酸或是α-酮戊二酸。